- TLEILAXU: Led by a small council of Tleilaxu Masters – the fanatic, xenophobic Tleilaxu were tolerated because of their useful genetic engineering superiority. Although underestimated and loathed by others, they hoped to someday dominate all.
- IXIAN: The cyborg, Prince Rhombur of House Vernius, leads the Ixians, masters of manufacturing and technologies only they know how to deploy.
- Not a complete game. You will need a copy of DUNE01 Dune: a Game of Conquest, Diplomacy & Betrayal to utilize this expansion.